Part:BBa_K3500005

pBI121-GUS

GUS gene which encodes the enzyme glucuronidase has been added to DinoIII plasmid which is specific for Dinoflagellate as a reporter gene. GUS gene is a good reporter gene in experiments that are conducted on plants, the success of transformation can be observed via the visual color change for the first time by applying a histochemical technique, termed GUS staining, to the transformants. In addition, DinoIII is a plasmid peculiar to Dinoflagellate such as Symbiodinium, which contains the original promoter belongs to Dinoflagellate, which to a certain extent will benefit heterologous gene expresses.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 542
  Illegal NgoMIV site found at 590
  Illegal NgoMIV site found at 722
  Illegal NgoMIV site found at 854
  Illegal NgoMIV site found at 1451
  Illegal NgoMIV site found at 1883
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 368
  Illegal BsaI.rc site found at 1403
  Illegal BsaI.rc site found at 1499
  Illegal BsaI.rc site found at 1787
  Illegal SapI.rc site found at 2083